Chemoenzymatic synthesis of CD52 glycoproteins carrying native N-glycans
2005
Abstract A facile synthesis of homogeneous CD52 glycoproteins carrying native N-glycans was achieved using an endolycosidase-catalyzed oligosaccharide transfer as the key step. The synthesis consists of two steps: the solid phase synthesis of GlcNAc-CD52 and the transfer of a high-mannose type or complex type N-glycan from Man 9 GlcNAc 2 Asn or a sialglycopeptide to the GlcNAc-CD52, under the catalysis of the endo-β- N -acetylglucosaminidases from Arthrobacter (Endo-A) and Mucor hiemalis (Endo-M), respectively.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
20
References
39
Citations
NaN
KQI