Biosynthesized Zinc oxide nanoparticles (ZnO NPs) using actinomycetes enhance the anti-bacterial efficacy against K. pneumoniae

Abstract Nanoparticles include a varied range of particulate materials, which exhibit at least one of its dimensions in the order of 1-100 nm. Biological methods are commonly employed for nanoparticle synthesis of which microbes play a major role owing to their cost efficiency when compared to the existing chemical and physical methods. Nanoparticles have unique physical, chemicals and optical properties than that of their bulk or commonly found forms. The study here elaborates on the potential of biologically synthesized zinc oxide nanoparticles from actinomycetes species isolated from the rhizosphere of soil in India. Zinc oxide is hexagonal in shape and of permanent white colour. Actinomycetes are commonly found gram positive bacteria in soil but appear like fungi with powdery mass. Innthis study catinomycete genus Streptomyces sp. was used to synthesize the zinc oxide nanoparticles (ZnONPs). In this study of synthesized nanoparticle was used to various biomedical application antibacterials and anti-biofilms against pathogenic microbes. Firstly, UV-spectrometer was used to analyse the ZnO NPs, which exhibited its resonance between 350-400nm. The studies of the biosynthesized ZnO NPs against multi drug resistant K. pneumoniae revealed 200µg/mL as Biofilm inhbition BIC with 88% biofilm degradation potential and 92% hydrophobicity index due to the effect of ZnO NPs influence.