In-house validation of a totally aqueous voltammetric method for determination of diltiazem hydrochloride

Abstract In this paper, diltiazem hydrochloride (DTZ) was electrochemically determined on cathodically pretreated boron-doped diamond electrode. The sustainable method showed potential of application using two supporting electrolytes, evaluating two different oxidation peaks of the drug. For voltammetric determination of DTZ, all operating parameters of square-wave voltammetry were optimized and analytical curves were constructed. The voltammetric method was in-house validated in terms of linearity, limit of detection, limit of quantification, precision, accuracy and selectivity. The pharmaceutical samples were analyzed by the official method and the results were statistically similar to those obtained by the voltammetric method. The totally aqueous voltammetric procedure was also applied to the determination of DTZ in biological fluids with excellent recovery values.