Sensor Array Based on Carbon Dots for ATP-related Physiological Phosphates Detecting and ATP Hydrolysis Monitoring

Abstract A simple indicator displacement assay (IDA)-based array system was established by using Fe(III)-responsive fluorescent carbon dots (CDs) and Fe(III) to detect ATP-related physiological phosphates (PPs) and monitor ATP hydrolysis. The analysis was based on principal component analysis of differential CDs fluorescence regulated by Fe(III)/PPs complexes. By two modeling methods, two sensor arrays were established. That is, three CDs/one Fe(III) concentration (Array1) and one CD/four Fe(III) concentrations (Array2). Compared with Array1, Array2 saved a lot of trouble in preparing the CD sensors and the analytical performance it is hardly affected. The Array2 can distinguish PPs in serum environment and from interfering substances, such as amino acids and metal ions. Besides, quantitative analysis of ATP single-component system and qualitative analysis of ATP/ADP two-component system can be achieved. On this basis, ATP hydrolysis processes were monitored, and results suggested that ATP hydrolysis completed within 20 min under the catalysis of 24 U/mL apyrase. As ATP hydrolysis works for 30 minutes, its main products were mainly AMP and orthophosphate (Pi).