Development and validation of RP-HPLC method for qunatitative analysis of Amlodipine besylate in pure and Pharmaceutical formulations

A Validated simple, sensitive, specific and precise RP-HPLC method was developed for the determination of Amlodipine besylate in pure and pharmaceutical formulations. Method was carried on Zodiac C18 column (250mm×4.6mm×5μ particle size) using Methanol: Acetonitrile: 0.01M Phosphoric acid (75:23:2) as mobile phase. Detection was carried out by U.V at 236nm. The proposed method obeyed linearity in the range of 10–100μg/ml and met all specifications as per ICH guidelines. Statistical analysis revealed that this method can be used in routine quality control studies of Amlodipine besylate in pure and its formulations.