Protective action of effective components of Huanglian Jiedu decoction on hypoxia and reoxygenation injury in cultured rat cerebral microvascular endothelial cells

Objective: To investigate the protective effect of geniposide,baicalin and berberine for the rat cerebral microvascular endothelial cell.Method: The model of hypoxia and reoxygenation injury in rat cerebral microvascular endothelial cells in vitro was established.Both normal and model cells were treated with geniposide(1.024,0.512,0.256,0.128,0.064,0.032,0.016,0.008 μmol·mL-1),baicalin(0.224,0.112,0.056,0.028,0.014,0.007,0.003 μmol·mL-1) and berberine(0.192,0.096,0.048,0.024,0.012,0.006,0.003 μmol·mL-1).Cell activity was measured by methyl thiazolyl tetrazolium(MTT) test.Result: After hypoxia/hypoglycemia cultures for 4 hour and reoxygenation for 12 hour,geniposide(0.128,0.064,0.032 μmol·mL-1),baicalin(0.028,0.014,0.007 μmol·mL-1) and berberine(0.024,0.012,0.006 μmol·mL-1) could protect the injuried cerebral microvascular endothelial cells.Conclusion: Appropriate concentration of geniposide,baicalin and berberine,which are effective components of Huanglian Jiedu decoction,could protect the injuried cerebral microvascular endothelial cells.