Research on opto-mechatronic biological microscope design

Abstract The monitoring method of 1–40 layer cell factories is limited in China. In order to observe the planting status of cell in the cell factories in a real-time, in-situ and efficient way, we propose an observing method which adopts to tilt observation cells through transparent side-wall of cell factories and it will improve the production efficiency of vaccines and other biological medicine. An Cassegrain reflective long working distance microscope objective has been designed so as to monitor the cultured cells in the cell factories. The magnification is 5 and working distance is 85 mm. The numerical aperture can reach 0.3 and the minimum distance resolution is 1.1 μm. At the same time, a servo-control system has been researched by utilizing single neuron adaptive PI control algorithm which aimed at the observation device. The servo-control system has high control precision and good repeatable performance. The system can fill the blank of domestic technology and have a great market prospect.