GS0202菌产人参皂苷－β-葡萄糖苷酶条件及其酶的反应条件

Production of the ginsenoside-β-glucosidasebybacteria GS0202 was optimized. The medium composition is proteose peptone 10 g, yeast powder 5 g, NaCl 10 g. 3.2 mg/mL total saponins of Panax ginseng and water 1 L and culture temperature is 28℃. The opimum temperature for enzyme catalysis is 25℃, pH 4 and the concentration of substrate is 3 mg/mL.