Expression Analysis of CsPLt Gene in Tea Plant [Camellia sinensis(L) O.Kuntze] by qRT-PCR

The cDNA-AFLP was applied to identify genes expressed differentially in cold acclimatized tea plant.A cDNA fragment,TDF6 encoding a polyol transporter was isolated.The cDNA fragment had 77 %,76 %,75 %homology with polyol transporter genes from Populus trichocarpa,Hevea brasiliensis and Lotus japonicus by BLAST on NCBI,and named CsPLt.qRT-PCR analysis showed that CsPLt gene were up-regulated by cold,dehydration,NaCl,the maximum expression were 7.3,12.2,5.3 times higher than that before treatment,respectively.For different tea organs,transcriptional level of the CsPLt gene was the highest in bud,next was in flower,the third was in root and stem,and higher than in mature leaves and the lowest in seed.It was deduced that CsPLt gene was very important for tea plant in response to abiotic stress and was strictly controlled in expression of different organs.