Leishmanicidal activity of Brosimum glaziovii (Moraceae) and chemical composition of the bioactive fractions by using high-resolution gas chromatography and GC-MS

As part of our ongoing SisBiota CNPq/FAPESP and Biota-FAPESP bioprospecting programs, this paper deals with the leishmanicidal properties of Brosimum glaziovii (Moraceae), and used gas chromatography analysis to determine the chemical composition of these fractions. The extracts and fractions from the leaves and branches of B. glaziovii were screened against Leishmania amazonensis. The hexane fractions from the leaves and branches displayed the highest leishmanicidal activities, with IC50 = 3.6 and 39.1 µg mL -1 . Using gas chromatography analysis it was possible to identify the sterols campesterol, stigmasterol, and b-sitosterol, as well as the triterpenes α-amyrin, b-amyrin, b-amyrin acetate, and lupenone, and other nonpolar components, mainly fatty acids and their derivatives. This is the first report on the leishmanicidal activity and the chemical composition of B. glaziovii.