Rapid detection of Campylobacter jejuni by multiplex PCR technique

Objective:To establish a multiplex PCR technique for rapid and specific detection of Campylobacter jejuni.Methods: Two sets of primers from mapA and hipO gene of Campylobacter jejuni were selected and added into one amplification system to perform PCR.The system was optimized,the specificity and sensitivity of this system were evaluated,and artificially-contaminated chicken were detected.Results: The assay was designed to amplify the 589 bp and 323 bp regions of corresponding genes mapA and hipO with high sensitivity and specificity.Sensitivity of the assay was 105 cfu/ml for bacteria samples and 101 cfu/ml for Campylobacter jejuni in artificially-contaminated chicken with incubation at 42℃ for 36 hours.Conclusion: A rapid,specific and sensitive multiplex PCR technique for the simultaneous detection of Campylobacter jejuni has been studied primarily.