Method for preparing (2S, 3R)-2-benzoyl aminomethyl-3-hydroxy methyl butyrate

The invention discloses a method for preparing (2S, 3R)-2-benzoyl aminomethyl-3-hydroxy methyl butyrate. The method comprises the following steps: preparing carbonyl reductase gene-containing engineering bacterium and glucose dehydrogenase gene-containing engineering bacterium; respectively preparing the resting cell suspensions of two engineering bacteria; mixing two resting cell suspensions of two engineering bacteria, mixing a substrate racemic 2-benzoyl aminomethyl-3-carbonyl methyl butyrate, a hydrogen donor and a cofactor, and performing an asymmetric reduction reaction to prepare the (2S, 3R)-2-benzoyl aminomethyl-3-hydroxy methyl butyrate; wherein a base sequence of the carbonyl reductase gene is shown as a SEQ ID NO.1, and the base sequence of the glucose dehydrogenase gene is shown as a SEQ ID NO.2. The method is capable of catalyzing the substrate racemic 2-benzoyl aminomethyl-3-carbonyl methyl butyrate to react for generating (2S, 3R)-2-benzoyl aminomethyl-3-hydroxy methyl butyrate, and can increase the conversion rate and purity of the products.