Pseurotin A Isolated from the Polygonumi cuspidatum Endophytic Fungus CB50 and Its Activity Assay

Objective:The strain endophytic fungus CB50 isolated from the stem of Polygonum cuspidatum was identified.The broth of CB50 was isolated.And the product was assayed for its antibacterial activity and antitumor activity.Method:The strain CB50 was identified by ITS sequence analysis.The product of CB50 broth was identified with 1H-NMR,13C-NMR,DEPT-90,DEPT-135,HMBC and HMQC.And the antibacterial activity was assayed with Klebsiella pneumonia and Bacillus subtilis,et al.The antitumor activity was assayed with A549 cells,et al.Result:Strain CB50 was isolated from the stem of Polygonumi cuspidatum and it was identified as Penicillium brevicompactum.Pseurotin A isolated from the broth of CB50 has the antibacterial activities to Klebsiella pneuomonia and Bacillus subtilis.And in addition,it has definite inhibition activity to the cell A549.Conclusion:Pseurontin A was firstly isolated from endophytic fungus of Polygonumi cuspidatum.And Pseurontin A has the antibacterial activities to Klebsiella pneuomonia and Bacillus subtilis and also the inhibition activity to the cell A549.