芡实对糖尿病肾病大鼠肾组织MMP-9、TIMP-1及Collagen IV表达的影响

Objective:To investigate the effect of Euryale ferox on the expression of MMP-9, TIMP-1 and CollagenⅣin renal tissues of diabetic ne-phropathy rats. Methods:65 Wistar male rats were used. 10 rats were selected randomly as the normal control group ( N) , the rest of 55 rats were made DN models by injected streptozocin into abdominal cavity ( STZ) , then these rats were randomly divided into 5 groups:diabetic nephropathic model group ( DN) , l-ow-dose Euryale ferox treatment group ( EL) , medium-dose Euryale ferox treatm-ent group ( EM) , high-dose Euryale ferox treatment group ( EH) and losartan pot-assium group ( LP) , and 11 rats in every group, while rats were treated respecti-vely by intragastric administration for 12 weeks with the different dose of Eur-yale ferox and losartan potassium. Meanwhile rats of group N and group DN r-eceived the same volume dis-tilled water by gavage. 24 h urinary protein, serum BUN, Scr, Urinary albumin excretion ratio ( UAER) and the urinary protein to creatinine ratio ( Up/Ucr) were measured after the research. The renal pathologica-l changes were observed by hematoxylin-eosin, Masson and Periodic acid Schif-f (PAS) staining. The expression of MMP-9, TIMP-1 and Collagen Ⅳ were det-ected by im-munohistochemistry. The gene expressions of MMP-9, TIMP-1 and Collagen Ⅳ were measured by Real-time PCR. Results:Compared with group N, the renal glomerular became hypertrophic and the extracellular matrix accum-ulated in group DN. 24 h uri-nary protein, BUN, Scr, UAER and Up/Ucr also in-creased significantly in group DN (P<0. 05);DN group has a decrease of mR-NA of MMP-9 but an increase of the TIMP-1 and Collagen Ⅳ expressions (P<0. 05). While the pathology changes were re-lieved, 24 h urinary protein, BU-N, Scr, UAER and Up/Ucr as well as the mRNA of MMP-9 increased but the decrease of the TIMP-1 and Collagen Ⅳ expressions in group EM, EH and L-P (P<0. 05). Conclusion:Euryale ferox may be affected by the expression of MMP-9 and TIMP-1 in the DN rats kidney, to reduce the extracellular matrix accumulation, thus it may relieve glo-merular sclerosis and decrease proteinuria, meanwhile protect kidney function and delay the progress of diabetic nephropath-y.