In vitro studies on antimutagenicity of water, alcoholic and oil extracts of Semecarpus anacardium

Objects: To test the antimutagenecity of water alcoholic and oil extracts of Semecarpus anacardium. Methods: Water, alcoholic and oil extracts were prepared and tested in the bacterial test system using Salmonella typhimuriurn strains TA96 and TA100 in the presence and absence of metabolic activities. The antimutagenecity was tested versus Benso(a)pyrene (BzP) in dose dependent manner. Alcoholic and water extracts were also tested in human lymphocyte in vitro for its antimutagenic activity versus BzP at concentration of 1OOO'g per tube. Results: The water, alcoholic and oil extracts of Semecarpus anacardium were antimutagenic versus BzP in a dose dependent manner. The water and alcoholic extracts were antimutagenic versus BzP induced micronuclei at concentrations of 1OOO'g per tube in human lymphocytes. Conclusion: Water, alcoholic and oil extracts of S. anacardium are antimutagenic against BzP induced mutagenecity.