On the deposition of phospholipids onto planar supports with the Langmuir—Blodgett technique using factorial experimental design 2. Optimizing lipid composition for maximal adhesion to platinum substrates

Abstract Mixtures of biologically important lipids were transferred, using the Langmuir—Blodgett technique, as an ordered monolayer and investigated regarding the ability to remain attached to a platinum surface. The composition of the mixture was varied in a systematic way, using a factorial, central composite design. Phosphatidic acid and phosphatidyl-ethanolamine, which when studied separately gave good adhesion to the support, were exchangeable in the mixtures. The proportion of phosphatidylcholine was more crucial. Concentration domains were found, with phosphatidylcholine present in significant amounts, within which the deposited film still had good adhesion properties. Cholesterol, also in critical proportions, contributed to the stability of the transferred monolayer.