Infection of WSSV shows potential promise of a novel antiviral amino-nitrophenyl medicine for application in culture of shrimp seedling

Abstract Due to no treatment available to interfere with the unrestrained outbreak and spread of white spot syndrome virus (WSSV), the virus results in a serious economic losses in shrimp industry. Here, a novel amino-nitrophenyl coumarin derivative 2-amino-4-(4-nitrophenyl)-5-oxo-4H, 5H-pyrano[3,2-c]chromene-3-carbonitrile (C3007) was evaluated for anti-WSSV effect in Litopenaeus vannamei larvae. The data demonstrated that WSSV replication was suppressed over 95% in the presence of 10 mg/L C3007 for 72 h infection. As a response, the mortality of WSSV-infected larvae was decreased more than 50%. After WSSV infection for 24 h, continuous updated immersion of C3007 resulted in the mortality further reduced additional 20%. Additionally, the data that horizontal transmission in a cohabitation was significantly inhibited by C3007 revealed may reduce the risk of virus transmission in shrimp seedling culture. We believe that C3007 is suitable for inhibiting WSSV transmission in static aquaculture systems. These results suggested C3007 can be used as a potential agent to treat and prevent WSSV infection in aquacultural shrimp industry.