An Analysis on Smad 4 Gene Mutation in CNE2 Cell Line

Objective:To investigate the mutation of Smad 4 gene in CNE2 cell line.Methods:Human Smad 4 gene cDNA sequence was obtained from CNE2 cell by using RT-PCR technique,and compared with Smad 4 gene sequences obtained from normal nasopharynx tissue and GenBank.Results:No Smad 4 gene mutation was found in CNE2 cells.Conclusions:CNE2 cell is a kind of malignant tumor without mutation of Smad 4 gene.So,it is suggested that Smad 4 gene may not be possibly a suspectable factor for human nasopharyngeal carcinomas.The recombinant plasmid containing Smad 4 gene developed in this study lays a base for the functional research of Smad 4.