Molecular Cloning of Gene Encoding for Lipoprotein in Streptomyces Lividans

A lipoprotein contains both proteins and lipids water-bound to the proteins including enzymes, transporters, structural proteins, antigens, adhesins and toxins. In the present study, a LpnS gene encoding lipoprotein from Streptomyces lividans 66 was cloned by using polymerase chain reaction, southern blot and hybridization. After sequencing, the LpnS gene had 1235 bps coding 415 amino acids that had 100% homology to lipoprotein of Streptomyces lividans TK 24 and 99 % of Streptomyces coelicolor A3(2) and 71 % Streptomyces flavogriseus ATCC 3331 and 71% of ABC transporter of Streptomyces griseoflavus. This gave more data about lipoprotein in Streptomyces lividans 66.