EFFECTS OF HALOGENATED HYDROCARBONS AND ELECTRO- MAGNETIC FIELD ON THE BONE TISSUE

The formation and eventually the functionability of the osteocytes are determined by the modifications of extracellular environment. The structure of bone tissue is changed after the hexachlorobenzene (HCB) or electromagnetic-field (EMF) treatment. The chemical potential of HCB is low, because this agent is table molecule. From these originate the biological effects of HCB: eg. deponation in different organisms and biological food-cascade. The biological effects of EMF have dependent from the energy content, frequency, wave-length. These environmental effects (chemical and/or physical) play a very important role among the factors that induce osteoporosis. We investigated, how can the extremely-low doses (eld) HCB diet and eld-EMF effect the bone by chronic treatment? Wistar rats (male, 100250g/bw.) were treated orally with chlorobenzenes (HCB: 0,5 ppm/bw. kg) for 30 and 90 days through gastric tubes. The HCB pretreated rats were treated by eld-EMF (50 mT, 60Hz) for further 30 days. The experimental samples were taken from the bone (femur) tissues of the treated, control and solvent-treated control animals. Tissue structure and Ca2+ content of the bone and the liver function ( γGT, SGOT, SGPT) and structure (morphometry) were investigated. As a result of the chronic chlorobenzene treatment (90 days) significant changes in the femur were detected. This modification of bone tissues was correlated to significant change in the Ca2+ content of the bone matrices. After the combined treating (HCB pretreating + eld-EMF) were checked the femur samples (same as the earlier), these showed no difference between the treated and controls. The eld-EMF alone did not caused significant changes in the examined parameters. From our results we suppose that the EMF treatment can defend the degenerative effects of HCB on the bone.