Production of monoclonal antibody against Salmonella typhimurium by hybridoma technique; Pembuatan antibodi monoklonal terhadap Salmonella typhimurium dengan teknik hibridoma

In this research S.typhimurium killed by irradiation was used as antigen was prepared by exposing the bacteria to gamma rays from {sup 60}Cobalt source with the dose of 2.5 kGy, Specific lymphocyte cell were obtained by immunizing 3 months old Balb-C mice with the antigen. the immunizations were done by subcutan route with the interval of 2 weeks. The hybridoma cells were made by fussing the specific lymphocyte cells with the myeloma cells. It was found that the animals (immunization + irradiation with a low dose of I Gy ) yielded monoclonal antibody with higher value (5.15 mg/ml) than the control animals (3.25 mg/ml). (author)