The effect on growth of some polymorphic restriction sites in porcine growth hormone gene locus

A 506bp segment of the porcine growth hormone gene encompassing base +206 of the first intro through base +711 of the third exon was amplified by PCR[Base numbering followed the designation of Vice Wells (1987)] . Two polymorphic restriction sites for ApaⅠ,MspⅠ were identified at +295～+300,+563～+566 respectively. DNA was from Jianquhai breeds?The effect of genotype on bodyweight was analysed .The result showed that there was no difference of bodyweight between G1G2 and G2G2 genotype pigs in MspⅠ polymorphic restriction sites. But in Apa Ⅰ polymorphic restriction sites, the 70-days-bodyweight and average day gain from birth to 70-days of G4G4 genotype pigs was extremely significant( P 0.01) higher than G3G4 /G3G3 genotype pigs.Result suggested that GH gene may be a candidate gene for quantitative trait locus in pigs.