Optimization of the resistance-boosting effect of lotus root starch using response surface method in production

The resistance-boosting technology of lotus root starch was optimized by response surface analysis. Through the preparation of experiments,the best concentration of starch milk was determined 8%,then the ranges of the dose of pullulance,proteolytic enzyme time and acid enzyme time were confirmed through single factor experiment. Using response surface methodology,it is respectively concluded that the dose of pullulance has a significant influence on the rate of resistance starch,but proteolytic enzyme time impact the smallest. And the optimal parameters were as follow:the dose of pullulance 45.1ASPU/g dry starch,proteolytic enzyme time 58.2min and acid enzyme time 3.04h. Under the conditions,the content of resistant starch respectively was 12.3%.