SAT0511 CYTOKINES, CHEMOKINES AND INFLAMMATORY BIOMARKERS IN NEUROSARCOIDOSIS

Background: Sarcoidosis is characterized by granulomatous inflammation, which in rare cases can affect the central nervous system, neurosarcoidosis (NS). Objectives: The aim of this study was to estimate levels of cytokines, chemokines, and vascular biomarkers in patients with NS. Methods: In this observational, cross-sectional study, cerebrospinal fluid (CSF) and plasma were collected from biopsy-proven sarcoidosis patients with clinical suspicion of NS. They were categorized into either a NS group (n=14) or a non-NS group (n=5) depending on fulfilment of NS criteria. The results were compared to a healthy control group (HC, n=11). Samples were analysed with Electrochemiluminescence, Mesoscale®. Results: The level of INF γ was significantly higher in NS group compared to non-NS group in CSF (median 11.3 pg/mL vs. 3.3 pg/mL) and in plasma (median 21.6 pg/mL vs. 4.5 pg/mL) In CSF and not in plasma, the level differs significantly in NS group compared to non-NS group, IL12/IL23p40 (median 54.05 pg/mL vs. 3.61 pg/mL), IL16 (median 8.7 pg/mL vs. 4.3 pg/mL), IL17A (median 1.37 pg/mL vs. 0.19 pg/mL), TNF β (median 0.37 pg/mL vs. 0.02 pg/mL), IL8 (median 55.4 pg/mL vs. 37.8 pg/mL), CCL11 (median 36.1 pg/mL vs. 16.4 pg/mL, CCL26 (median 6.7 pg/mL vs. 3.7 pg/mL), CXCL10 (median 4981 pg/mL vs. 771 pg/mL), CCL13 (median 24.5 pg/mL vs. 10.0 pg/mL), CCL22 (median 129.8 pg/mL vs. 22.6 pg/mL), CCL3 (median 54.1 pg/mL vs. 20.7 pg/mL), CCL17 (median 54.0 pg/mL vs. 6.9 pg/mL), ICAM1 (median 13901 pg/mL vs. 7327 pg/ml), and VCAM1 (median 18594 pg/mL vs. 12132 pg/mL) A cut-off level for each cytokine was set at 20% above the maximum values of both non-NS group and HC. Using this, the ratio of patients in NS group over were: -INF γ, 57% had level over 6.2 pg/mL in CSF, and 50% had level over 21.6 pg/mL in plasma. -IL12/IL23p40, 71% had level over 16.7 pg/mL in CFS. -CXCL10, 79% had level over 1614 pg/mL in CFS. -CCL22, 79% had level over 49.8 pg/mL in CFS. Conclusion: In NS patients, INF γ was elevated in both CSF and plasma, and multiple cytokines, chemokinves and vascular biomarkers were elevated in CSF. Disclosure of Interests: Keld-Erik Byg: None declared, Zsolt Illes: None declared, Tobias Sejbaek Grant/research support from: Biogen, grants, outside the submitted work. , Consultant of: Personal fees from Novartis, outside the submitted work. , Astrid Kindt: None declared, Torkell Ellingsen: None declared, Helle Nielsen: None declared