Tissue culture and rapid propagation of dwarf-late pomelo.

Aseptic seeding of dwarf-late pomelo were used as the materials in the study,We induced cespitose buds from meristem and epicotyl of the seedlings,then regenerated plants from the induced shoots,and results showed that both mature and immature seeds could be germinate on 1/2MS and MS medium,the highest germination rate could reach 96%,and the aseptic seeding from mature seeds were more suitable for the further differentiation.The epicotyl was found to be the optimal explants,the optimal protocol for cespitose buds proliferation was to culutre upicotyl on MS medium with 2.0 mg·L~(-1) 6-BA,0.1 mg·L~(-1) NAA and 40 g·L~(-1) sucrose,and the achieved multiplication coefficient was 8.4.The optimal rooting medium was 1/2MS medium with 0.2 mg·L~(-1) NAA,0.2 mg·L~(-1) IBA 0.2 g·L~(-1) AC,from which 90%rooting rate was obtained.After ransplanted to nutrition bowl,80%plants survived.