Physiology Activity of Barley Leaf Using Different Drying Methods

Institute of Korean Food Research and Development, National Academy of Agricultural Science, RDA, Gyeonggi 441-853, KoreaAbstractThis study was to analyze the physiology activity of barley leaf extract using different drying methods. Yield of RL (raw leaf) and barley leaves dried did not show significant difference by various drying methods. There was no significant difference in total phenolic content by dry method. However, total flavonoid content was high in HD (dried after heat treatments) barley leaf after RL. There was no significance in barley leaf dried by SD (dried in the shade), FD (vacuum freeze dried) and MW (dried by microwave) after the microwave. Superoxide dismutase-like activity was high over 90%: 95.6±0.3% in RL, 94.9±0.7% in HD barley leaf, 92.0±1.3% in SD barley leaf, 91.5±0.4% in FD barley leaf, and 92.5±0.2% in MW barley leaf using the microwave. Significantly higher antioxidant activity was shown as compared to the control group of sesamol (88.426±0.802%), tocopherol (88.8±0.6%), and BHT (86.6±0.8%). Also, except for RL and MW barley leaf, all showed over 80% peroxyl radical scavenging activity and over 90% inhibition rate of xanthine oxidase. The results of this study show that total phenolic content and total flavonoid content by dry method were dependent on the drying temperature with no impact on antioxidant activity.Key words: barley leaf, drying, flavonoid, SOD-like activity, xanthin oxidase, peroxyl radical