Ascaris lumbricoides: alteration of the erythrocyte superficial charge using the Partition Method in aqueous two-phase system

Sialic acid is responsible for the negative charge of the erythrocyte. The decrease of sialic acid has hemodynamical and hemorheological importance. The aim was to study the effect of A. lumbricoides on the erythrocyte superficial charge using the Partition Method in aqueous two-phase system in order to indirectly evaluate the alteration of sialic acid in the red cells. We worked with five parasite extracts (AE) and larvae concentrate (LC). Erythrocyte superficial charge was studied by working with non-treated (Controls) and treated erythrocytes. The treatment consisted of incubating the erythrocytes with AE or LC for 30 minutes at 4 oC, 20 oC and 37 oC. The red cells were separated in a sensitive charge two-phase system (Dx/ PEG). The partition coefficient (P) of treated and untreated erythrocytes were calculated. The results showed a P decrease at the three temperatures for red cells treated with four of the AE. The remaining extract did change P values at any of the temperatures studied. The erythrocytes treated with LC showed a decrease in the P value at 37 oC and 4 oC but no change was observed at 25 oC. Statistical analysis concluded that P values were significantly lower in treated erythrocytes than in their corresponding untreated ones (p La disminucion de acido sialico, responsable de la carga negativa del eritrocito, tiene importancia hemodinamica y hemorreologica. El objetivo fue estudiar el efecto de A. lumbricoides sobre la carga superficial eritrocitaria aplicando el metodo de particion en sistemas bifasicos acuosos, a los fines de evaluar de manera indirecta la alteracion de acido sialico de los eritrocitos. Se trabajo con 5 extractos del parasito adulto (EA) y con un concentrado de larvas (500-600 larvas/mL) (CL). Se estudio la carga superficial eritrocitaria, trabajando con eritrocitos no tratados y tratados. El tratamiento consistio en la incubacion de los eritrocitos con EA o CL durante 30 minutos a 4 oC, 25 oC y 37 oC. Los eritrocitos fueron sometidos a la separacion en un sistema bifasico carga sensible constituido por Dx / PEG. Se calculo el coeficiente de reparto (P), de los eritrocitos sin tratar y tratados. Los resultados mostraron disminucion de P a las 3 temperaturas, en hematies tratados con 4 de los EA. El EA restante no modifico los valores de P a ninguna de las temperaturas estudiadas. CL produjo la disminucion de P a 37 oC y 4 oC, pero no se observo modificacion a 25 oC. Los analisis estadisticos concluyeron que los valores de P son significativamente menores en los eritrocitos tratados que en los respectivos eritrocitos sin tratar (p