Trichoderma sp efficient spore-production medium and culture method thereof

2015 
The invention discloses a trichoderma sp efficient spore-production medium and a culture method thereof, and relates to a spore-production medium and a culture method thereof. The invention aims to solve the problem of low spore output, which limits the production and application of trichoderma sp preparation. The spore-production medium is composed of culture matrix, mineral elements, and sterile water, and the culture matrix is composed of corn particles, rice husk, and wheat bran. The method for culturing trichoderma sp comprises the following steps: adding the trichoderma sp efficient spore-production medium into a triangular flask, wherein the volume of the added culture medium is 8% of that of the triangular flask; inoculating trichoderma sp liquid into the culture medium, wherein the inoculation amount is 35%, the concentration of the trichoderma sp liquid is 106 spores/mL, and the culture temperature is 28 DEG C. The provided medium has the advantages of simple components, low cost, and simple preparation method. The spore output of green trichoderma sp M1 and M2 can be prominently increased, and can reach 6.5*10 to 1.8*10 cfu/g. The provided medium and method are used to produce green trichoderma sp.
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