EFFECTS OF LOW-LEVEL OZONE EXPOSURE ON REACTIVITY AND CONDUCTANCE IN GUINEA PIG AIRWAYS

Previous studies have shown airway hyperreactivity in guinea pigs induced by ozone concentrations from 1 to 4 ppm for periods of up to 4 h. Ambient ozone levels only rarely exceed 0.2 ppm in megacities such as Mexico City and normally lie below 0.1 ppm. Here we investigate the effects of an ozone concentration of between 0.3 and 0.45 ppm upon airway function and inflammatory cell influx in guinea pigs. Airway function was monitored in conscious guinea pigs over a 24-h time course following a 2-h ozone exposure. A significant bronchoconstriction was seen as a decrease in specific airway conductance (sG) at 0.25, 0.5, and 4 h postozone, with a recovery to basal aw values after 7 h, which lasted for the following 17 h. Airway reactivity to methacholine and 5-hydroxytryptamine (5-HT), both iv and inhaled, was assessed in anesthetized guinea pigs between 2 and 3 h after an ozone exposure of the conscious animal. A clear ozone-induced airway hyperreactivity was observed as a leftward shift of the doseresponse c...