Study on in vitro fast-propagation and industrialization breeding techniques of triploid Chinese white poplar

The tissue culture and industrialization breeding techniques were studied by adopting the stem segment of single bud of Populus tomentosa one-year-growth seedling. The study results indicated that the proliferation and radication of in vitro seedling were made simultaneously, the common-used culture medium was H+NAA 0.15 mg/L and radication rate was 80%~98%. And it proliferated 8 times annually and its propagation index was 5; The medium of vermiculite + turf with 1∶1 proportion was best for transplanting and training of Populus tomentosa in vitro seedling. Through production of in vitro seedling and trial of seedling planting in warm shed and open field, craft process of industrialization breeding of Populus tomentosa was put forward in its 3 continuous courses of inoculation, cultivation, transplant and nursery garden management stages in this paper.