Effect of Fenton pretreatment and bacterial inoculation on cellulose-degrading genes and fungal communities during rice straw composting

Abstract The aims of this article were to study the effect of Fenton pretreatment and bacterial inoculation on cellulose-degrading genes and fungal communities during rice straw composting. The rice straw was pretreated by Fenton reactions and functional bacterial agents were then inoculated during the cooling phase of composting. Three treatment groups were carried out, the control (CK), Fenton pretreatment (FeW) and Fenton pretreatment and bacterial inoculation (FeWI). The results indicated that Fenton pretreatment and bacterial inoculation changed the fungal communities composition and increased fungal diversity, leading to changes in the cellulose-degrading genes. In addition, a network analysis showed that in the FeWI treatment, the fungi from modules 1, 5 and 8 were core hosts of the cellulose-degrading genes driving the cellulosic degradation. Moreover, Fenton pretreatment and bacterial inoculation changed the core module fungal communities and strengthened the correlation between the core fungi and the cellulose-degrading genes, thereby promoting cellulosic degradation. Based on redundancy and structural equation model analyses, the NH4+-N, TOC, pH and Shannon index were important factors influencing the variations in the cellulose-degrading genes. This study provides a foundation for cellulosic degradation during cellulosic waste composting.