Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean

RAV is a member of the ERF/AP2 transcription factor family.We use explants of Arabidopsis rav T-DNA insertion homozygous mutants and inhibition(-i)of GmRAV in soybean as experimental material to carry out callus induction.Various concentration of exogenous Cytokinins 2-ip and 6-BA were added in the shoot-inducing medium and the changes in the average number of shoots and the shoot regeneration rates were investigated.It turned out that Cytokinins had positive effects on the shoot regeneration,in addition,the average number of shoots and shoot regeneration rates of Arabidopsis rav mutants and GmRAV-i soybean plants would decrease by 2-ip and 6-BA.It proved that the function of RAV homologs was conserved and RAV promoted shoot regeneration in the presence of Cytokinins.