cydA, spdC and mroQ are novel genes involved in the plasma coagulation of Staphylococcus aureus.

Coagulation is a critical pathogenic pattern in Staphylococcus aureus (S. aureus). Although the agglutination of S. aureus has been studied for a long time, the genes involved in this are not completely clear. We adopted tube agglutination and dynamic turbidimetry tests to identify novel genes involving reduced plasma coagulation. There were 15 genes had been identified, including coa, clfA, vwbp, saeS, agrA, trpC, spdC, mroQ, cydA, qoxC, sucC, pyrP, menH, threonine aldolase and truncated transposase for IS1272. These genes have different functions and map onto functional agglutination, two-component regulatory, membrane transfer, energy metabolism and biosynthesis, etc. cydA, spdC and mroQ genes were further studied by gene knockout and complementation. Results of gene knockout and complement and RT-qPCR proved that cydA, spdC and mroQ genes were necessary in plasma coagulation. Furthermore, 7-day mice's survival ability decreased significantly when cydA, spdC and mroQ genes had been knocked out. This article is protected by copyright. All rights reserved.