Introducing samples directly into electrospray ionization mass spectrometers by direct infusion using a nanoelectrospray interface.

Procedures are described for constructing and using a microscale electrospray interface for direct infusion of samples into mass spectrometers. The sensitivity of the nanospray interface is a result of greatly reducing the flow of sample solution while preserving the analyte signal intensity. The described methodology provides a simple and robust way to analyze individual purified peptide and protein samples, i.e., samples that do not require liquid chromatography separation. Curr. Protoc. Protein Sci. 74:16.8.1-16.8.7. © 2013 by John Wiley & Sons, Inc. Keywords: mass spectrometry; nanoelectrospray; microscale electrospray interface