A m6A sensing method by its impact on the stability of RNA double helix.

: N6-methyladenosine (m6A) is one of the most important RNA modifications in epigenetics.  The development of detection method for m6A is limited by its abundance and structure.  Although it has been previously reported that its presence has an impact on the complementary pairing of RNA, few assays have been developed using this finding. We used this discovery and designed a detection method based on cas13a system, which has different fluorescence signals for target RNAs containing m6A modification and target RNAs without m6A modification. We verified the fact that the presence of m6A could cause the instability of dsRNA using the Cas13a system and provided a new direction and strategy for the development of m6A detection methods in the future.