Cage-Shape Macroporous/Mesoporous 3DOM SiO2 Immobilized Papain

In this research, cage-shape macroporous/mesoporous three-dimensional-ordered material (3DOM) SiO2 was prepared using polystyrene colloidal crystal (PS) as template and TEOS as silica source, in which papain was immobilized. The optimal immobilization, the stability and the application of the immobilized papain were studied. Compared with the free papain, the immobilized papain exhibits higher activity. The thermal and pH stability of the immobilized papain obviously improved.