Features of Stress-Induced Changes of HSP70 Expression in Populations of Immunocompetent Cells

The results of intracellular protein measurement carrying out with the use of monoclonal antibodies, strongly depend on the localization of antibody binding epitopes in the structure of the molecular target. Our previous study has shown pronounced differences in the pattern of cellular stress response detected by monitoring of intracellular HSP70 content, which was assessed using flow cytometry and monoclonal antibodies interacting with various domains of these molecules. In the present work we investigate the features of stress-induced changes in the expression of HSP70 in populations of mononuclear and granulocytic cells from human peripheral blood using standard methods: PCR, Western-blot and flow-cytometric analysis. A particular feature of this study is an application of a number of antibodies including monoclonal antibodies specific for the total pool of HSP70, separately for the inducible or constitutive form of this protein, as well as for the different domains of the HSP70 molecule. The results provide new additional information on HSP70-related stress-induced processes in populations of immunocompetent cells.