Enantioselectivity-promoting factor in enzyme-mediated asymmetric hydrolysis of enol esters

Abstract Enantioselectivity-promoting factor enhances enantioselectivity of protonation in lipase AP-catalyzed asymmetric hydrolysis of enol esters. The factor was partially purified by chromatography using Phenyl-TOYOPEARL 650M and Sephacryl S-200HR. The hydrolysis of 2-benzyl-l-cyclohexenyl acetate by PLE in the presence of the purified factor produced ( R )-2-benzylcyclo-hexanone in 92% ee, while the reaction without the factor gave the racemate.