Microwave‐assisted extraction (MAE) combined with gas chromatography‐mass spectrometry (GC‐MS) for determination of volatile small molecule to evaluate compatibility of antimicrobial peptide PL‐5 spray with packaging materials

A simple and efficient method involving microwave-assisted extraction (MAE) combined with GC-MS was established to determine 1,3-di-tert-butylbenzene (DBB), 2,4-di-tert-butylphenol (DBP), 3,5-di-tert-butyl-4-hydroxybenzaldehyde (DBHBA) for evaluating the compatibility of antimicrobial peptide PL-5 spray with drug-packaging materials. In this study, the antimicrobial peptide PL-5 spray was purified using a Welchrom C18 column, and the high-density polyethylene spray bottle with liquid collection tube was first mixed with absolute ethanol, which would be subjected to MAE for further measurement by GC-MS. Various experimental parameters were systematically optimized, and good linearities were obtained within the range of 0.05-1.00 mug/mL for DBB and DBHBA and 0.50-10.00 mug/mL for DBP, with limits of detection (LODs) of 0.99 ng/mL (DBB), 16.34 ng/mL (DBP), and 1.64 ng/mL (DBHBA). Satisfactory recoveries that ranged from 96.0% to 107.2% were acquired, and the relative standard deviation was