1141222326 Analysis of effects of androgen on protein profiles in TM4 mouse Sertoli cells by SELDI‐TOF mass spectrometry

Aim:  The identification of proteins induced by androgen in Sertoli cells is a major issue in spermatogenesis. Methods:  In this study, we analyzed protein profiles in TM4 Sertoli cells treated with dihydrotestosterone using SELDI-TOF mass spectrometry. Results:  We found increases in expression of a 5.0 kDa protein at 15 min, a 11.3 kDa protein at 24 hr and 4.3, 5.7, 5.8, 9.95 and 9.98 kDa proteins at 48 hr. On the other hand, expression of 6.3 and 8.6 kDa proteins were decreased at 30 min, and 4.9, 5.0, 12.4 and 19.8 kDa proteins at 48 hr. The 11.3 kDa molecule was identified as macrophage migration inhibitory factor (MIF) known to having various functions. The 9.98 kDa molecule was identified as calgizzarin related to calcium channel. The timing of its expression suggests that MIF and calgizzarin are involved late in androgen regulation of spermatogenesis in Sertoli cells. The 19.8 kDa molecule was identified as translationally controlled tumor protein (TCTP) known to bind to tublin and Ca2+ and regulates cell proliferation. Conclusion:  Androgenic effects on spermatogenesis are exerted eventually through MIF, calgizzarin and TCTP.