Heterologous expression in yeast of fungi polyketide synthesis gene

The present invention is, from genes containing introns with eukaryotic, to remove introns contained in the gene linked only exon sequences, a method of making an expression vector containing the linked sequences. Specifically, from the huge gene mold containing introns were amplified by PCR using exon sequences as one or more fragments, using the gap repair cloning method concatenates the fragments and restriction enzyme treated vector, ligated exon a method for preparing an expression vector comprising the sequence, giant a cDNA fragment of the gene was ligated synthesized and a method of making an expression vector containing the full-length cDNA sequence, a transformant is expression vector prepared by the method was introduced, proteins produced by transformant, and to a method of obtaining a compound produced by the protein using the expression vector.