HCL-05 : High throughput and Mass Producible Microstructure Platform for Rare Tumor Cell selection

Background Circulating tumor cells (CTCs) play an important role in estimating the presence and the metastatic relapse of tumor. Despite of their importance, isolation of viable CTCs is still struggling, since chemical or mechanical damages are unavoidable when separating less than 1000 of CTCs out of billions of other blood components. Furthermore, the current CTC isolation devices show low productivity, since they are produced after a series of complicated fabrication processes. Methods Here, we present a low-cost and mass-producible microstructrue filters for the viable CTC isolation and the further molecular assay for profiling cancer-associated markers. Results By utilizing size-based sorting method, the microstructrue filter is capable to isolate both epithelial and mesenchymal CTCs, while slots with curved walls are beneficial for preventing the cell rupture by reducing 21.6% of mechanical stress compared to the conventional straight walled slots. We applied our filter to 11 human blood samples and found that the number of CTCs was closely related to the expression level of Ki-67, which is highly overexpressed in proliferative tumors. Conclusion The microstructrue filter might be an appropriate caner-screening tool in developing countries, where people suffer from insufficient healthcare services.