Detection and partial characterization of new polypeptides in peanut cotyledons associated with early stages of infection by Aspergillus spp.

Polypeptide profiles of peanut cotyledonary tissue from viable kernels of 14 cultivars grown under normal irrigation and five genotypes grown under drought stress were determined before and after invasion by Aspergillus flavus and A. parasiticus. Inoculated kernels and isolated cotyledons were removed from moist chambers every 6 hr within 48 hr after inoculation. Polypeptide patterns were determined by microprocessor-controlled sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis and visualized after staining with silver (...)