Presence of catecholamine-related enzymes in a subpopulation of primary sensory neurons in dorsal root ganglia of the rat

: The presence of enzymes (tyrosine hydroxylase (TH) and dopamine-beta-hydroxylase (D beta H)) and enzymatic activities (monoamino oxidase, (MAO)) related to catecholamine synthesis and degradation have been investigated in cervical, thoracic and lumbar dorsal root ganglia (DRG) of adult male rats using immunohistochemical and enzyme histochemical techniques, respectively. A small population (between 2-4%) of TH-like immunoreactive and MAO positive neurons was found. They were small in diameter (18 +/- 2 microns), predominate in lumbar DRG and did not display D beta H-like immunoreactivity. These sensory neurons which are likely catecholaminergic were insensitive to systemic administration of capsaicin and 6-hydroxydopamine. Colchicine administration caused an increase of TH-like immunoreactivity and MAO activity. Pargyline produced an increase in TH-like immunoreactivity and the disappearance of MAO activity. The possible dopaminergic nature of the subpopulation of DRG sensory neurons investigated in the present study is discussed.