Preparation method and detection method for biguanide drug residue detection kit

The present invention relates to a preparation method and a detection method of a biguanide drug residue detection ELISA kit, wherein the biguanide drug residue detection ELISA kit has characteristics of sensitive, accurate and rapid detection, simple operation and strong specificity, and is suitable for detecting large batch samples. The kit comprises an enzyme label plate coated with biguanide drug antigen, a biguanide drug standard substance, a biguanide drug antibody working liquid, a biguanide drug enzyme labeled second antibody working liquid, a substrate liquid A, a substrate liquid B, a termination liquid, a concentrated liquid dilution liquid and a concentrated washing liquid. The principle of the biguanide drug detection kit is a solid phase indirect competitive enzyme-linked immunoassay reaction, wherein the extracted sample, the enzyme-labeled secondary antibody working liquid and the antibody working liquid are added to the corresponding enzyme label cells, incubating is performed for a certain time, the plate is washed, the substrate liquid A and the substrate liquid B are added, the blue color appears in the cell under the enzyme effect, the termination liquid is added, the color is changed into the yellow color from the blue color, and the color depth and the biguanide drug content in the standard substance or sample form the inversely proportional relationship. The method of the present invention can be used directly for detecting the biguanide drug in the health products or cosmetics.