PHOSPHORYLATION OF Ser-359 OR Ser-370 PRECEDES PHOSPHORYLATION AT OTHER SITES AND IS REQUIRED FOR ACTIVITY*

proteins fails to trans-locate to the plasma membrane when the cells are stim-ulated. In contrast, normal phosphorylation and trans-location are seen in mutants containing aspartate orglutamate at positions 359 and 370, but oxidase activityis still greatly reduced. These results imply that a nega-tive charge at position 359 and/or 370 is sufficient toallow the phosphorylation and translocation of p47