Electrospun Nanofibers with Core–Shell Structure for Treatment of Bladder Regeneration

Hyaluronic acid (HA) is an important component of the extracellular matrix (ECM), and electrospun scaffolds have the 3D porous network structure characteristics similar to those of the autologous ECM. However,scaffolds fabrication of water dissolved HA without surfactant by electrospinning is difficult. This study reports the fabrication of HA-coated biomimetic nanofibrous scaffolds via coaxial electrospinning of poly(L-lactide)/poly(e-caprolactone) (PLCL) and HA to modulate proliferation and migration of smooth muscle cells (SMCs) for bladder regeneration. The nanofibers were verified by scanning and transmission electron microscopy, ATR-FTIR spectroscopy. Contact angle and swelling were measured to determine hydrophilicity of the nanofiber scaffolds. SMCs were seeded onto the PLCL/HA and PLCL scaffolds for the in vitro study. For the in vivo study, 36 rats were divided into the PLCL/HA, PLCL, cystotomy controls and sham surgery groups,8 rats per group. The 40–50% supratrigonal cystectomy was excised and...