Therapeutic Effects of Three Human-derived Materials in a Mouse Corneal Alkali Burn Model

AbstractPurpose: To compare the therapeutic effects of human derivatives in a mouse alkali burn model.Methods: The right eyes of mice were injured using NaOH. After alkali injury, one of the following agents was topically administered for 7 days: human amniotic membrane (hAM) suspension, human umbilical cord serum (hUCS), and human peripheral blood serum (hPBS), or saline. The epithelial defect areas on days 1, 2, and 3, degrees of opacity on days 2, 3, and 7, and corneal neovascularization (NV) areas on day 7 were evaluated. Histologic examination and mRNA expression levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-2, MMP-8, and MMP-9 were also evaluated on day 7.Results: The epithelial defect areas in the hUCS group were smaller than those in the control and hPBS groups on day 3 (p < 0.05, respectively). The epithelial defect areas in the hAM suspension group showed smaller than those in the control and hPBS groups on ...