Knockout of csrA Gene in Escherichia coli and Its Effects on Phenylalanine Biosynthesis

csrA(carbon storage regulator A) gene encodes a global regulatory protein relating to central carbon metabolism in E.coli aromatic pathway.The global regulatory gene csrA was disrupted by using Red recombinant system,and confirmed by PCR and DNA sequencing methods.Comparing with the control strains,in csrA-disrupted strain the rate of conversion from glucose to amino acid increased and the L-phe production also increased by nearly 13%.