Antibiotic resistance pattern of urinary isolates in a Rural Medical College of Maharashtra

Objective: To determine resistance pattern of urinary isolates in a rural medical college of Maharashtra. Methods: Urine samples were collected using midstream clean catch method in clinically diagnosed UTI cases from august 2016 to February 2017. A total of 320 samples were analyzed under supervision of qualified microbiologist. Antibiotic sensitivity of isolated microorganisms were tested for commonly used antibiotics by Kirby bauer technique. Results: Out of 320 samples 200 were positive. The pathogens isolated were E.coli 60 (30%), Pseudomonas 45 (22.5%), Methicillin resistant Staphylococcus aureus 35 (17.5%) & Klebsiella 30 (15%). E.coli being the most common isolate. The isolates showed high degree resistance for Amoxycillin & Cotrimoxazole. Conclusion: 62.5% of samples showed significant growth. Moderate yield suggest moderate clinical correlations in suspected cases of UTI. E.coli showed low level resistance to Nitrofurantoin (1.67%), Amikacin (3.34%), Norfloxacin (13.3%) & high degree resistance to Cotrimoxazole (63.34%), Piperacillin + Tazobactam (50%), fluoroquinolones (27.04%). It’s very clear from the study E.coli is the MOC infecting uropathogen & developing resistance against the commonly used antibiotics. Keywords: Uropathogens, Antibiotic resistance, Urinary tract infections